Productos Bio-rad

Ofrecemos equipos de la marca Bio-rad líder mundial en el desarrollo, fabricación y comercialización de productos para los mercados de investigación de ciencias biológicas y diagnóstico clínico.

Bio-rad Proteómica

Bio-Spin® 6 and Micro Bio-Spin™ 6 Columns
Más Información

Bio-Spin 6 and Micro Bio-Spin 6 columns clean protein samples quickly using size exclusion chromatography. Filled with Bio-Gel P-6 gels, these columns are shipped fully hydrated in Tris buffer and are ready to use. They effectively clean and remove salts from protein samples in just 10 minutes.

Bio-Spin 6 and Micro Bio-Spin 6 columns:

  • Provide fast salt and contaminant removal in an easy-to-use spin-column format
  • Remove compounds <6 kD by size exclusion chromatography
  • Accommodate up to 100 µl of sample
ReadyPrep™ Reduction-Alkylation Kit
Más Información

The ReadyPrep reduction-alkylation kit reduces and irreversibly blocks the formation of inter- and intramolecular disulfide bonds prior to isoelectric focusing (IEF). Disulfide bond formation is particularly problematic for basic proteins because of the increased rate of their formation in an alkaline environment. Moreover, many reducing agents become negatively charged during IEF and migrate off the IPG strip, thus allowing disulfide bonds to re-form (Görg 2000, Hoving 2002).

The ReadyPrep reduction-alkylation kit:

  • Eliminates the potential for disulfide bond formation during IEF
  • Reduces disulfide bonds with tributylphosphine (TBP) and alkylates the sulfhydryl groups with iodoacetamide (Herbert et al. 2001)
  • Provides reagents for treatment of 100 samples (300 µl each, containing up to 10 mg/ml of protein)
  • Performs treatment directly in the rehydration/sample buffer, in less than 2 hours, and with very little hands-on time
  • Is compatible with other ReadyPrep sample preparation kits and buffers

References
Görg A et al. (2000). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 1,037–1,053.
Herbert B et al. (2001). Reduction and alkylation of proteins in preparation of two-dimensional map analysis: Why, when and how? Electrophoresis 22, 2,046–2,057.
Hoving S et al. (2002). Preparative two-dimensional gel electrophoresis at alkaline pH using narrow range immobilized pH gradients. Proteomics 2, 127.

ReadyPrep™ Sequential Extraction Kit
Más Información

The ReadyPrep sequential extraction kit is used to extract proteins of differing solubility from cell lysates. Based on the work of Molloy et al. (1998) and Herbert et al. (1998), the kit uses a series of three extraction buffers, each differing in their detergent and chaotrope concentrations (Rabilloud et al. 1997). The insoluble residues from each extraction step are further extracted with reagents of increasingly stronger solubilizing power.

The ReadyPrep sequential extraction kit:

  • Yields fractions that are suitable for direct analysis by a number of methods, including 2-D electrophoresis
  • Can be carried out in a single microcentrifuge tube, minimizing protein loss
  • Results in manageable 2-D protein patterns for simplified protein identification and gel matching
  • Generates fractions with high protein loads to enable visualization of low-abundance proteins

 

References
Molloy, MP, et al. (1998). Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19, 837–844.
Herbert, BR, et al. (1998). Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis 19, 845–851.
Rabilloud, T, et al. (1997). Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307–316.

ReadyPrep™ Protein Extraction Kit (Soluble/Insoluble)
Más Información

The ReadyPrep protein extraction kit (soluble/insoluble) separates total protein samples into two fractions based on differential protein solubility. It generates a soluble fraction containing hydrophilic proteins and an insoluble fraction containing hydrophobic proteins. Proteins in the insoluble fraction are eventually resolubilized in ReadyPrep 2-D rehydration/solubilization buffer 1, a strongly chaotropic solution containing the zwitterionic detergent ASB-14. This simple fractionation strategy results in less complex samples and leads to improved identification of low-abundance proteins and a better overall view of the proteome.

ReadyPrep™ Protein Extraction Kit 
Más Información

The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

The ReadyPrep protein extraction kit (cytoplasmic/nuclear):

  • Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989)
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that may interfere with IEF

References
Dignam JD et al. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acid Res 11, 1475–1489.

Zerivitz K and Akusjarvi G (1989). An improved nuclear extract preparation method. Gene Anal Tech 6, 101–109.

ReadyPrep™ Protein Extraction Kit (Membrane I)
Más Información

The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.

The ReadyPrep protein extraction kit (membrane I):

  • Isolates proteins associated with membranes or containing two transmembrane domains
  • Does not require the use of ultracentrifugation or density gradients
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF

 

References
Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.

 

ReadyPrep™ Protein Extraction Kit (Membrane II)
Más Información

The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al. 1982, Molloy et al. 2000) to fractionate and isolate membrane proteins.

The ReadyPrep protein extraction kit (membrane II):

  • Isolates complex proteins that contain more than two transmembrane domains or those that are resistant to extraction by the ReadyPrep protein extraction (membrane I) kit
  • Requires ultracentrifugation
  • Includes a strongly chaotropic, IEF-compatible rehydration/sample buffer that contains the detergent ASB-14 to efficiently solubilize proteins

 

References
Fujiki Y et al. (1982). Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biology 93, 97–102.
Molloy MP et al. (2000). Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem 267, 2871–2881.

ReadyPrep™ Protein Extraction Kit (Signal)
Más Información

The ReadyPrep protein extraction kit (signal) employs differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts. These proteins are often involved in intracellular membrane trafficking and signaling pathways and include the following: glycosylphosphatidylinositol (GPI)-anchored proteins, caveolin, caveolae (caveolin-associated proteins), acylated tyrosine kinases, heterotrimeric G proteins, and some proteins with transmembrane domains. These proteins are resolubilized in the protein solubilization buffer (PSB), which contains nondetergent sulfobetaine 201 (NDSB 201) along with urea, thiourea, and CHAPS, and which is compatible with a variety of downstream applications, including IEF.

Aurum™ Ion Exchange Mini Kits
Más Información

Descripción

Aurum ion exchange mini kits utilize ion exchange chromatography to selectively enrich either acidic or basic proteins in less than 30 minutes. These easy-to-use kits include the columns and buffers for cation exchange (CEX) or anion exchange (AEX) chromatography and can be used with a variety of starting samples. Isolated proteins are suitable for most downstream proteomic applications, including 2-D gel analysis and mass spectrometry.

 

Aurum CEX

Aurum AEX

Type of support

UNOsphere S

UNOsphere Q

Binding Capacity

12 mg protein/column*

36 mg protein/column*

Column bed volume

0.2 ml

0.2 ml

Binding buffer

20 mM sodium acetate, pH 5.0

20 mM Tris, pH 8.3

Elution buffer

Binding buffer + 1.0 M NaCl

Binding buffer + 1.0 M NaCl

* Binding capacity determined using IgG (CEX) and BSA (AEX). Capacity will differ slightly depending on the protein.

 

Aurum ion exchange mini kits:

  • Use a patented* polymerization technology to achieve ultrahigh protein binding capacity
  • Accommodate up to 1 ml of sample
  • Use a quick, easy, spin-column format
  • Work on a variety of sample sources (serum, plasma, mammalian tissues or cells, plant leaves, E. coli, yeast)

*U.S. patent 6,423,666.

ProteoMiner Protein Enrichment Kits
Más Información

ProteoMiner protein enrichment technology employs a large, highly diverse bead-based library of combinatorial peptide ligands, which simultaneously dilutes high-abundance proteins and concentrates low-abundance proteins. When a complex biological sample is applied to the beads, high-abundance proteins saturate their high-affinity ligand(s) and excess protein is washed away. In contrast, low-abundance proteins are concentrated on their specific affinity ligand(s).

ProteoMiner protein enrichment kits:

  • Decrease the amount of high-abundance proteins without immunodepletion, preventing the loss of proteins bound to high-abundance proteins.
  • Enrich medium- and low-abundance proteins that cannot be detected through traditional methods.
  • Do not rely on a predefined set of antibodies, unlike immunodepletion products.
  • Are compatible with a variety of sample types.
  • Offer a convenient, easy-to-use format.
  • Can be used for differential expression analysis.

 

Multiple Kits Available
A small-capacity kit is available for processing ≥10 mg of protein, while a large-capacity kit is available for processing ≥50 mg of protein. These kits have been optimized for use with serum and plasma samples, but they may be used with other sample types within the recommended total protein range.

The small- and large-capacity kits are available in two formats:

  • ProteoMiner protein enrichment kits — provide all necessary reagents for accessing low-abundance proteins, utilizing a single elution reagent in a simple, easy-to-perform process.
  • ProteoMiner sequential elution kits — utilize multiple elution reagents to sequentially elute proteins based on different properties. These kits fractionate proteins to improve detection and resolution and have been optimized for SELDI experiment samples (reagents are not compatible for direct use with 2-D gel electrophoresis).
Aurum Affi-Gel Blue Mini Columns and Kit
Más Información

Aurum Affi-Gel Blue mini columns effectively remove up to 90% of albumin from serum or plasma samples by affinity chromatography.Albumin removal reduces protein load by 70–80%, allowing the application of 34 times more serum and significantly improving theresolution of polypeptide spots on 2-D gels. Columns are available individually in packs of 25 or 100, or with buffers as part of the Aurum Affi-Gel Blue mini kit.

Aurum Affi-Gel Blue mini columns:

  • Consist of Micro Bio-Spin columns filled with 100–200 mesh Affi-Gel Blue
  • Use a quick and easy spin-column format
  • Provide eluted proteins ready for IEF analysis in less than 30 minutes
Aurum Serum Protein Mini Kit
Más Información

The Aurum serum protein mini kit enables selective binding and simultaneous removal of both albumin and IgG from serum or plasma samples. These two proteins can mask the presence of many underlying smaller proteins that are similar in size, limiting the amount of serum that can be resolved on a 2-D gel or by other applications. The Aurum serum protein mini kit was specifically designed to maximize resolution of proteins of interest, greatly improving and supporting protein discovery and identification.

The Aurum serum protein mini kit:

  • Consists of Micro Bio-Spin columns filled with a mixture of Affi-Gel Blue and Affi-Gel protein A support
  • Easily and effectively removes albumin and IgG by affinity chromatography
  • Uses a quick and easy spin-column format
  • Provides eluted proteins ready for IEF analysis in less than 30 minutes

Because the Aurum serum protein mini kit utilizes Cibacron Blue technology, some nonspecific binding of other serum proteins may occur. However, this can add an extra dimension to your analysis. All proteins bound to the column can be resolved with a simple elution using ReadyPrep sequential extraction reagent 3.

PROTEAN® i12™ IEF System
Más Información

The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.

Features and Benefits of the PROTEAN i12 IEF System

  • Optimize experiments in fewer runs — the PROTEAN i12 IEF system has the unique ability to run channels under multiple conditions in a single run. Different samples, protocols, and pH gradients can be run simultaneously.
  • Obtain higher-quality data with less experimental risk — each channel is independent, so one irregular sample can no longer compromise the entire run.
  • Easy, intuitive programming — easily run, create, and edit isoelectric focusing protocols from the touch screen user interface. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample-specific IEF conditions.
  • Run gel side up, gel side down, or with loading cups — focusing trays and electrode assemblies accommodate all possible gel configurations.

Applications and Uses of the PROTEAN i12 IEF System

Isoelectric focusing is the first dimension of 2-D electrophoresis, a powerful technique used for the visualization and identification of proteins. The PROTEAN i12 IEF system can be used for a number of applications including the following:

  • 2-D electrophoresis and analysis
  • Protein profiling — analysis of complex protein mixtures
  • Biomarker discovery — discovery/identification of specific targets and markers

Functional proteomics — structure, interactions, and function

 

Trans-Blot Turbo Transfer System
Más Información

TRANS-BOLT TURBO TRANSFER SYSTEM

The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The Trans-Blot Turbo Transfer Packs include an optimized buffer, membrane, and filter paper combination that provides superior blot transfers when paired with the Trans-Blot Turbo blotting apparatus. Protein blotting consumables are available in a ready-to-use, pre-packaged stack or in bulk quantities that require assembly.

Features and Benefits of the Trans-Blot Turbo Transfer System

  • Rapid blot transfer — 3 min transfers of mini or midi gels
  • High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
  • Greater transfer efficiency — higher transfer efficiency than other protein blotting methods
  • Flexible design — allows the user to customize transfer conditions and is compatible with traditional semi-dry consumables
  • Environmentally friendly — environmentally safe consumables eliminate disposal cost
  • Recommended for Stain-Free Western Blotting — permits a streamlined workflow

Trans-Blot® SD Semi-Dry Transfer Cell
Más Información

The Trans-Blot® SD semi-dry transfer cell allows fast, efficient, economical blotting without a buffer tank or gel cassettes.

Features and Benefits

  • Transfers take place in as little as 15–60 min
  • Minimal buffer requirements save money
  • Capacity to transfer up to four Mini-PROTEAN® precast or handcast gels, or three Criterion™ gels
  • Single-step locking system allows simple, speedy setup
  • Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
  • Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
  • Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

  • Western blotting
  • Nucleic acid transfer
  • Northern and Southern blot transfers in 10–35 min
  • Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Bio-rad Genómica

PCR CFX Opus
Más Información

PCR CFX Opus

CFX Opus es el sistema de PCR en tiempo real más nuevo de nuestra sólida familia CFX. El sistema de PCR en tiempo real CFX Opus 96 puede analizar 96 pozos y ofrece una serie de características nuevas:

  • Rendimiento térmico más uniforme
  • Conectividad ampliada: Wi-Fi, Ethernet y USB
  • Conectividad en la nube: opere CFX Opus en nuestra plataforma en la nube BR.io o con el software CFX Maestro de escritorio
  • Acceso a la unidad de almacenamiento en red para una excelente gestión de datos
  • El sistema óptico Shuttle produce mediciones ópticas consistentes en su placa de muestra

Folleto del sistema CFX Opus

Folleto de investigación

Folleto de Biofarma

 

Fuente PowerPac
Más Información

La fuente de alimentación PowerPac Basic es ideal para electroforesis en gel sumergida horizontal y mini vertical y proporciona suficiente salida para la celda Mini Trans-Blot ® 

Utilice la fuente de alimentación básica PowerPac para aplicaciones tales como gel vertical mini y midi y electroforesis horizontal sumergida, y secando con la celda Mini Trans-Blot ® . La fuente de alimentación es totalmente programable para corriente o voltaje constante, temporizada o no temporizada.

Documentos y recursos de respaldo

 

Mini-Sub Cell GT Cell
Más Información

Mini-Sub Cell GT Cell
Wide Mini-Sub Cell GT Cell
Sub-Cell GT Cell

The redesigned Mini-Sub Cell GT Cell offers updated features that make electrophoresis even easier. The Mini-Sub Cell GT Cell can resolve up to 30 samples; its short, narrow format allows 7 and 10 cm runs with speed, simplicity, and economy.

Bio-Rad’s mini cells resolve EcoRI or HindIII digests of lambda DNA in only 1.5 hours at 60 V. Small DNA fragments can be separated in as little as 15 minutes at 150 V.

All mini cells accommodate ReadyAgarose Precast Gels to save time and allow highly reproducible separations, and include a buffer tank, a safety lid with cables, and a leveling bubble. System configurations that include additional accessories are also available (see the Mini-Sub Cell GT Complete Systems Selection Guide for more details).

Features:

  • QuickSnap* electrodes are easy to remove, simplifying cleaning
  • Arrow on the side of the base indicates the direction of the run and ensures proper orientation of the gel
  • Color-coded, labeled electrodes and labeled base guarantee correct positioning of the lid on the base
  • Tabs on the base permit easy removal of the lid, reducing buffer spillage, and also prevent incorrect lid positioning
  • Reverse-compatible design allows the cells to be used with components from older models
  • Clear plastic construction for easy sample visualization
  • UV-transparent gel trays with fluorescent ruler**
  • Gel-casting gates to cast your own gels right in the cell, or optional caster for tape-free casting**
  • Combs to fit every need (fixed-height drop-in combs, adjustable-height combs, and preparative combs)**

* Patent pending.
** Purchase separately for ReadySub-Cell GT systems.

Mini ReadySub-Cell GT Cells

The mini ReadySub-Cell GT cell is identical to the Mini-Sub Cell GT Cell, except it is dedicated to running ReadyAgarose precast gels. These gels lock securely into the mini ReadySub-Cell chamber, offering convenience and reproducibility. This cell does not include casting gates, tray, or combs. Kits are available to upgrade ReadySub-Cell GT Cells for handcasting capability.

Wide Mini ReadySub-Cell GT Cell

The wide mini ReadySub-Cell GT Cell is identical to the wide Mini-Sub Cell GT Cell, except it is dedicated to running ReadyAgarose Precast Gels. These gels lock securely into the wide mini ReadySub-Cell chamber, offering convenience and reproducibility. This cell does not include casting gates, tray, or combs.

* Patent pending.
** Purchase separately for ReadySub-Cell GT Systems.

CHEF Mapper
Más Información

The CHEF Mapper XA system incorporates patented FIGE and AFIGE* technologies for superior resolution in the range of 100 bp to 10 Mb, in addition to the features of the CHEF-DR II and the CHEF-DR III systems. The CHEF Mapper XA system also includes algorithms for deriving separation conditions. This system is ideal for both the PFGE novice and the seasoned expert.
Each CHEF Mapper XA system is supplied with a power module, embedded auto-algorithm for protocol optimization, interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, and accessory kit.

Automation
The CHEF Mapper XA system offers two ways to optimize your separations. The unique auto-algorithm can automatically select optimal separation conditions, integrating 11 key variables and implementing starting separation conditions. Protocols can be refined using the Windows operating system-based interactive algorithm, which lets you specify several run variables simultaneously to derive optimal separation protocols.

Customization
Busy laboratories need equipment that can store and readily access key separation conditions. The CHEF Mapper XA system can store up to 99 programs.

Application Versatility
The CHEF Mapper XA system is the most flexible of the PFGE units. The CHEF Mapper XA system achieves higher resolution with greater speed and accuracy than any other PFGE system, making it ideal for all PFGE applications. The CHEF Mapper XA system lets you choose any pulse angle from 0 to 360°, which allows optimal separation of both chromosomal and plasmid DNA with one system. Accurate sizing of fragments requires an expanded linear range of separation. Switch-time ramps increase the mobility of fragments by gradually changing the switch times during the course of a run. Nonlinear (for example, concave or convex) ramps change the switch-time increments from the start to the end of a run. These nonlinear ramps separate fragments linearly from 50 to 700 kb, yielding more precise fragment size measurements. The multistate mode of the CHEF Mapper XA system enhances resolution in selected fragment size ranges. Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). Up to eight different states can be combined into one run to optimize the separation of subsets of fragments in the sample. The application of secondary pulses of defined voltage, duration, angle, and frequency can enhance the separation and resolution of very large DNA molecules by releasing DNA caught in the gel matrix.
* U.S. patent 5,549,796.

VER FOLLETO

Colorante de carga UView ™
Más Información

UView 6x Loading Dye

UView 6x loading dye is a dual-action fluorescent nucleic acid stain and loading dye that allows for immediate visualization of DNA fragments after electrophoresis without the need for in-gel or post electrophoretic staining/destaining. While sensitive, it is also nontoxic and nonmutagenic, making it an attractive alternative to ethidium bromide.

UView 6x loading dye allows you to:

  • Save time with a combined DNA stain and loading dye
  • Visualize your DNA fragments immediately after electrophoresis
  • Use a nontoxic and nonmutagenic alternative to ethidium bromide
  • Detect samples in concentrations as low as 6 ng

The UView 6x loading dye is best for DNA electrophoresis applications.

T100 Thermal Cycler
Más Información

T100 Thermal Cycler

The T100 thermal cycler is a small thermal cycler offering a comprehensive set of convenient features in a small footprint. This compact thermal cycler features an intuitive touch-screen user interface to make running PCR easy. Thermal gradient technology allows you to quickly optimize your reaction in a single run. With its robust design, the T100 system is a reliable personal thermal cycler that delivers exceptional performance for years — Launch the System Tour to test drive the T100 thermal cycler now.

Features and Benefits of the T100 Thermal Cycler

  • Intuitive touch screen — easy programming using a large color touch screen and intuitive interface
  • Consistent results — robust design ensures effective and consistent performance run to run
  • Easy optimization — this gradient thermal cycler allows fast PCR optimization using a unique thermal gradient
  • Easy protocol management — protocols can be organized using personalized folders or a USB flash drive

Small space-saving footprint — the T100 is a compact thermal cycler that fits in any laboratory

Reliance Select cDNA Synthesis Kit
Más Información

Reliance Select cDNA Synthesis Kit contains a newly engineered reverse transcriptase designed to perform well with challenging samples and targets. This kit has the capability of processing samples with difficult inhibitors and degraded RNA (e.g., FFPE samples) as well as targets containing secondary structures and/or high GC content.

Features and Benefits

  • Novel engineered reverse transcriptase with superior performance — compatible with degraded samples such as FFPE, inhibitors and targets with high GC content
  • 10 minute reaction protocol — quickly and easily complete cDNA synthesis
  • High tolerance to common reaction inhibitors — enables higher reproducibility and consistent results, even in samples with inhibitor carry-over
  • High versatility — stock a single reverse transcription kit in the lab
  • Thermostability up to 55ºC allows for better performance with secondary structures
iScript cDNA Synthesis Kits
Más Información
  • 1-Step RT-qPCR from RNA or Cells

    Bio-Rad offers robust and reliable solutions to amplify RNA targets on all qPCR platforms including both ROX-dependent and ROX-independent systems. Our reagents feature:

    • Easy reaction set up with 1- or 2-tube formats
    • Sensitive, reproducible multiplex of up to 5 targets
    • Room temperature stability of assembled reaction up to 24 hours

    Wide instrument compatibilitytures

High-Fidelity PCR Kits
Más Información

iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.

Features and Benefits of iProof High-Fidelity DNA Polymerase:

  • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)

Choose the best DNA polymerase for your application.

iScript Select cDNA Synthesis Kit
Más Información
The iScript Select cDNA Synthesis Kit is a high-fidelity, sensitive reverse transcription system that provides ultimate flexibility. Unlike our other iScript cDNA Synthesis products, the reaction components for this kit are packaged in individual tubes, enabling flexible priming options and customization of reaction setup for specific application needs.

Key Features and Benefits

  • Achieve high-fidelity — unique reverse transcription kit minimizes introduction of base pair errors
  • Synthesize long cDNAs — efficiently synthesize cDNA greater than 8 kb
  • Save time and reduce errors during setup — less hands on time and less pipetting steps compared to protocols for other high-fidelity reverse transcriptase kits
  • Detect low-level target genes and conserve RNA — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase

Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription

Aurum™ Total RNA Mini Kit
Más Información

The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a spin-column format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications. The Aurum total RNA mini kit can also be used for RNA cleanup and desalting.
Features include:
• High-quality RNA isolation from a wide variety of samples in less than 60 minutes
• RNase-free reagents and plasticware, including DNase I for removal of genomic DNA, at no additional cost
• Rapid silica binding-based purification technology in a spin-column format
• Easy-to-use spin- or vacuum-compatible protocol
Genomic DNA, RNA, and Plasmid Purification Kits
Bio-Rad offers a complete line of products for the isolation and purification of nucleic acids. From the purification of milligram quantities of plasmid DNA for restriction analysis or transformation to the rapid and efficient purification of PCR products, Bio-Rad provides you with high-quality kits that eliminate time-consuming, labor-intensive steps.

Aurum™ Total RNA 96 Kit
Más Información
The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
Features include:
• High-throughput total RNA isolation in less than 60 minutes
• RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
• Rapid silica binding-based purification technology in a 96-well plate format
• Easy-to-use spin- or vacuum-compatible protocol
Aurum™ Total RNA Fatty and Fibrous Tissue Kit
Más Información

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt. The kit is perfect for purifications involving fatty and fibrous tissues or samples such as fungi that are rich in RNases. It also works well with most animal and plant tissues, cultured cells, yeast, and bacteria.
The Aurum total RNA fatty and fibrous tissue kit combines the best features of PureZOL™ RNA reagent, a potent phenol-based reagent that effectively lyses tissues and cells, with the speed of silica membrane technology in a spin-column format to yield high-quality, intact RNA in minimal time.
The entire purification process, including DNase I digestion, is completed in less than 60 minutes. The kit includes mini spin columns that can be processed using a choice of vacuum- or spin-mediated protocols. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
Features include:
• High-quality RNA isolation from difficult-to-process samples in less than 60 minutes
• RNase-free reagents and plasticware; includes DNase I for the removal of genomic DNA
• Rapid silica binding-based purification technology in a spin-column format
• Easy-to-use spin- or vacuum-compatible protocol

Quantum Prep® Plasmid Kits
Más Información

The consistent high yields achieved with Quantum Prep plasmid kits are due to the high-affinity binding of DNA to the unique Quantum Prep DNA binding matrix. This patented matrix, a special grade of diatomaceous earth prepared with a proprietary treatment to activate the largest possible surface area, is available only from Bio-Rad. The large surface area allows the use of enriched growth media, such as Terrific Broth, to obtain the highest yields. Quantum Prep kits use an easy spin-based procedure to quickly go from cell culture to purified plasmid DNA. Plasmid DNA is recovered in water or TE for immediate use in all downstream applications.
Features include:
• High purity and yield of plasmid DNA
• Easy spin-column procedure to significantly reduce plasmid DNA isolation time
• Stringent QC specifications ensure high-quality plasmid DNA for immediate use in all downstream molecular biology applications

Aurum™ Plasmid Mini Kit
Más Información

The easy-to-use Aurum plasmid mini kit improves the efficiency and throughput of plasmid purifications with a simple bind-wash-elute protocol using silica membranes. Lysed bacterial cultures can be cleared either by centrifugation or by vacuum filtration with the Aurum vacuum manifold. The clarified lysates are then applied to plasmid binding columns and plates, where DNA is bound, then washed, and finally eluted — all in <10 minutes. The purified plasmid DNA can be immediately used in any downstream molecular biology application.
The Aurum plasmid mini kit delivers high yields of reproducible plasmid DNA preparations for:
• Automated fluorescent sequencing
• Restriction digestion
• Ligation and transformation
For more information, request bulletin 2664.

InstaGene™ Matrix
Más Información

InstaGene matrix, made with a specially formulated 6% w/v Chelex resin, makes DNA sample preparation fast, easy, and cost-effective, providing PCR-quality template DNA in less than an hour. The Chelex matrix binds to PCR inhibitors rather than DNA, preventing DNA loss due to irreversible DNA binding. The InstaGene matrix eliminates the time-consuming and labor-intensive deproteinization, organic extraction, dialysis, and alcohol precipitation protocols required in traditional DNA purification procedures. Place the cells in a microcentrifuge tube, add the InstaGene matrix, boil, and spin. The matrix adsorbs cell lysis products that interfere with the PCR amplification process. DNA in the supernatant is ready for PCR.
Features include:
• Ease of use — the small-particle-size slurry won’t clog pipet tips and is easily resuspended with the included stirbar
• Appropriate matrix concentration ready upon resuspension
• Affordability and cost-effectiveness — 100 samples from 1 bottle

Chelex 100 Molecular Biology Grade Resin
Más Información

Chelex 100 Molecular Biology Grade Resin can be used for fast, easy nucleic acid preparations from many sample types including forensic, blood, parasites, viruses and bacteria. DNA and RNA prepared using Chelex 100 is suitable for PCR or RT-PCR.
Chelex 100 chelates polyvalent metal ions, with a selectivity for divalent over monovalent ions of approximately 5,000 to 1. The resin avidly binds divalent cations such as Mg2+, inactivating metallo-nucleases and other enzymes, as well as binding other polyvalent metal ions that interfere with enzyme-based applications such as PCR and ligation. Due to the high selectivity for divalent over monovalent ions, Chelex 100 Molecular Biology Grade Resin can be used for DNA and RNA preparations from samples with high levels of salts.
• Chelex 100 eliminates time-consuming and labor-intensive steps required with conventional nucleic acid extraction methods. A typical DNA or RNA extraction protocol involves: add Chelex 100 suspension to the sample, boil to release nucleic acids and denature sample, spin to pellet resin beads and sample debris, use supernatant for downstream steps such as PCR or RT-PCR.
• Molecular biology grade Chelex 100 Resin is certified to be endo- and exonuclease and ligase inhibitor-free.
• Larger volumes and special packaging are available upon request.

ReadyPrep™ Protein Extraction Kit (Total Protein)
Más Información

The ReadyPrep protein extraction kit (total protein) provides a simple, rapid, and reproducible method for preparation of total cellular protein extracts from a wide variety of biological samples. Use of this kit generates protein samples that can be applied directly to a variety of applications, including IEF and 2-D gel separation.
The ReadyPrep protein extraction kit (total protein):
• Includes a strongly chaotropic extraction solution containing the zwitterionic detergent ASB-14
• Provides reagents for up to 20 extractions, each using 50–100 mg of cells or tissue
• Can easily be scaled up to accommodate larger amounts of cells or tissue
• Generates total protein samples in about 45 minutes

MicroRotofor™ Cell Lysis Kits
Más Información

Find the best kit for your needs.
Use our Product Information Sheet. »

MicroRotofor lysis kits provide cell lysis and protein extraction protocols that are tailored to the specific needs of different sample sources. Convenient and easy to use, all four kits are based on the same chaotropic protein solubilization buffer (PSB), which contains nondetergent sulfobetaine 201 (NDSB 201) along with urea, thiourea, and CHAPS for particularly effective solubilization (Vuillard et al. 1995). The kits generate total protein samples that are ready for fractionation with the MicroRotofor liquid-phase IEF cell, but they may also be applied to other downstream applications, such as SDS-PAGE and 2-D gel electrophoresis.

Species-Specific Protocols
Different sample types have different requirements for effective cell disruption, and all four kits combine PSB with other elements to accommodate these specific needs.

  • MicroRotofor cell lysis kit (mammal) — uses maceration and solubilization (up to a 100 mg sample) using ReadyPrep mini grinders and PSB (Vuillard et al. 1995). The kit is intended for use with samples from mammalian sources, but it may also be applied to other animal tissues
  • MicroRotofor cell lysis kit (bacteria) — for use with both gram-negative and gram-positive bacterial cultures. It employs enzymatic digestion using lysozyme followed by solubilization into PSB (Vuillard et al. 1995)
  • MicroRotofor cell lysis kit (plant) — for use with soft plant tissues and cultured cells. It employs maceration (up to a 1 g sample) and solubilization into PSB. A ReadyPrep 2-D cleanup kit is provided to enable removal of plant-derived phenolics, which may interfere with IEF
  • MicroRotofor cell lysis kit (yeast) — for use with yeast cultures (~60 µl wet cell pellet). It employs enzymatic digestion of the cell wall with lyticase followed by solubilization into PSB (Scott et al. 1980, Vuillard et al. 1995)

References
Scott J et al. (1980). Lyticase: endogluconase and protease activities that act together in cell lysis. J Bacteriol 142, 414–423.
Vuillard L et al. (1995). Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification. Biochem J 305, 337–343.

Real-Time PCR Supermixes & Kits
Más Información

Real-Time PCR Supermixes and Kits

Bio-Rad’s supermixes can make any qPCR experiment easier, faster, and more
effective. Our real-time PCR supermixes are designed for:

  • Any instrument — universal reference dye is compatible with all qPCR platforms
  • Any chemistry — supermixes for SYBR Green or probe-based detection chemistry

Any conditions — our patented Sso7d fusion polymerase* guarantees superior qPCR performance under varying and/or challenging conditions.

cDNA Synthesis Kits
Más Información

Reverse transcription is a crucial step for gene expression studies, sequencing, cloning, and other applications. Optimization of this step is a priority to researchers to ensure the best results downstream. We offer a variety of cDNA synthesis kits to meet researchers’ needs, including high fidelity or dealing with challenging samples, difficult targets, and rare or long transcripts.

One-Step RT-qPCR Reagents & Kits
Más Información
High-Fidelity and Standard PCR 
Más Información

Standard PCR Enzymes and Reagents

iTaq DNA Polymerase is an antibody-mediated hot-start DNA polymerase suitable for both PCR and real-time PCR applications. The enzyme is activated after a 3 min denaturation step at 95°C. iTaq DNA Polymerase is highly specific, sensitive, and easy to use.

Features and Benefits of iTaq DNA Polymerase:

  • Improved product yields compared to non-antibody-based DNA polymerases
  • Amplification of a wide range of targets
ReadyPrep™ Mini Grinders
Más Información

ReadyPrep mini grinders are used in sample extraction protocols to grind small biological samples for high recovery of proteins and nucleic acids. Each mini grinder includes a 1.5 ml grinding tube containing a grinding resin and a matching pestle for effective disruption of cells and tissues. The mini grinders fit in most benchtop centrifuges.

ReadyPrep mini grinders are:

  • Convenient for small-scale extraction from biological tissues
  • Disposable and protease-free
  • Also sold as components of the MicroRotofor cell lysis kit (mammal)
ReadyPrep™ Reagents for Extraction and Solubilization
Más Información

A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation). Some of the premixed buffer and reagent components of these kits are also available separately for use in customized protein extraction and solubilization protocols.

ReadyPrep™ 2-D Cleanup Kit
Más Información

The ReadyPrep 2-D cleanup kit uses selective precipitation to remove ionic contaminants such as detergents, lipids, and phenolic compounds from protein samples. These contaminants may interfere with separation, particularly during IEF and 2-D separations. Use of the kit improves 2-D resolution and reproducibility. This kit can also be used to clean up samples prior to other applications in which contaminants may negatively impact results.

The ReadyPrep 2-D cleanup kit:

  • Employs a TCA-like protein precipitation to remove ionic contaminants from protein samples
  • Enables concentration of proteins from samples that are too dilute, allowing for higher protein loads that can improve spot detection
  • Can be used with virtually any protein sample
  • Reduces streaking, background staining, and other gel artifacts associated with ionic contaminants
  • Provides reagents for either 5 or 50 preparations
PCR DIGITAL QX200 
Más Información

PCR DIGITAL QX200

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR System

  • Most precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required

See performance data for the QX200 Droplet Digital PCR System. ddPCR instrument control and analysis are performed by QuantaSoft Software.

Watch our Success Stories videos to learn more about the applications of the Droplet Digital System in diverse research fields.

View our Droplet Digital PCR reference library with links to scientific journal articles, conference posters, BioRadiations articles and Bio-Rad technical reports.

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
    Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection
    Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
  • Next generation sequencing
    Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
  • Gene expression analysis
    Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
  • Environmental monitoring
    Test a wide variety of environmental samples like soil and water using the QX200 System.
  • Food testing
    Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:

    • Copy number variation analysis
    • Rare mutation and sequence detection
    • Gene expression
    • Next-generation sequencing library analysis

 

Droplet Digital PCR Technology and Workflow

The QX200 Droplet Digital PCR System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, and their associated consumables.

The QX200 Droplet Generator is used to partition ddPCR reaction mix into thousands of nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. Droplets are read well by well as they are streamed single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

Additional Instruments

The QX200 Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Bio-rad Imágen

ChemiDoc MP
Más Información

CHEMIDOC MP

The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Complete Capabilities: Superior Images

Stain-Free
Total Protein Normalization

Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
See Data »

Chemiluminescence
Replaces Film

Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
See Data »

Multiplex Fluorescence
3 Colors

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
See Data »

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores; see the fluorophore compatibility chart
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Request a Free Fluorophore Reference Poster

Determine the best fluorophore for maximum sensitivity and high signal-to-noise ratio in your next experiment using this poster.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi ChemiDoc MP Stain-Free Western Blotting also include:

  • ChemiDoc MP Imaging System
  • Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
  • PowerPac Basic Power Supply (1645050)
  • Trans-Blot Turbo Transfer System (1704150)
  • 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

ChemiDoc
Más Información

The ChemiDoc Imaging System provides fast, reliable, and sensitive imaging and documentation of gels and chemiluminescence western blots. This system is compatible with stain-free technology, chemiluminescence detection, and a wide range of gel stains such as ethidium bromide, SYPRO Ruby, Coomassie, and silver stains.

The ChemiDoc Imaging System can be upgraded to gain the full fluorescent western blotting capabilities of the ChemiDoc MP System. The upgrade package has an increased spectrum (RGB, far red, and near infrared), allowing multiplex fluorescence western blotting capability with minimal cross-talk between channels. Multiplex fluorescence allows detection of multiple antigens on the same blot, eliminating the need for stripping and reprobing.

Stain-Free Western Blotting provides efficient, consistent electrophoresis and blotting with the ability to rapidly assess electrophoresis and blot transfer quality, and perform total protein normalization.

ChemiDoc Imaging Systems

Complete Capabilities: Superior Images

Stain-Free
Total Protein Normalization

Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
See Data »

Chemiluminescence
Replaces Film

Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
See Data »

Multiplex Fluorescence
3 Colors

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
See Data »

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible chemiluminescence and colorimetric blot detection, analysis, and gel documentation in a single system
  • Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminate the need for housekeeping proteins
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi Gel Stain-Free Western Blotting include:

  • ChemiDoc Imaging System
  • Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
  • PowerPac Basic Power Supply (1645050)
  • Trans-Blot Turbo Transfer System (1704150)
  • 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)
ChemiDoc XRS+
Más Información

The ChemiDoc XRS+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Key benefits include:

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The ChemiDoc XRS+ System optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The ChemiDoc XRS+ System offers fast and sensitive chemiluminescence detection in addition to gel and blot documentation of fluorescent and colorimetric samples. The system includes a supersensitive 16-bit CCD camera that is supercooled for detection of faint samples. The ChemiDoc XRS+ system can be used for imaging with a wide variety of applications that require high resolution and sensitivity (such as chemiluminescent western blots).

The ChemiDoc XRS+ System eliminates the need to use costly and unreliable X-ray film technologies while providing quantitative and reproducable data in seconds. The system features a signal accumulation mode (SAM), which guides a user through determining optimum exposure time and capturing a desired image of a chemiluminescent sample.

GELDOC GO
Más Información
The GelDoc Go Gel Imaging System gives you a benchtop imaging solution in a compact and evolved package. Acquire high-resolution, publication-quality images of both nucleic acid and protein gels. Advanced optical design for a large imaging area allows you to image up to four mini gels at once but still retain a compact size. Easily set up and control the GelDoc Go with Image Lab Touch Software. Powerful to move your research forward. Small enough to fit anywhere.

The GelDoc Go Gel Imaging System features the intuitive Image Lab Touch Software onboard, with capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy.

Image Lab Touch Software Features:

  • Quickly set up the GelDoc Go System to capture your image, add notes, and export or print your files
  • Easily manage user accounts and set permissions and security options
  • Works with Image Lab Desktop Software for deep and accurate analysis of your data

Product Contents

  • GelDoc Go Gel Imaging System with Image Lab Touch Software
  • UV/stain-free imaging sample tray (additional imaging trays available separately)

Accessories for the GelDoc Go Imaging System

  • White Sample Tray for Coomassie Blue and other white-light stains (12012165)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12012160)
  • GelDoc Go UV Shield (12012164)
  • Holder for Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates (12012190)
  • GelDoc Go Imaging System IQ/OQ Kit (12012147)

ZOE Fluorescent Cell Imager7 
Más Información

ZOE FLUORESCENT CELL IMAGER7

The ZOE fluorescent cell imager is a complete imaging system that is suitable for routine cell culture and imaging applications. It eliminates the complexities of cell imaging by combining the ease of use of a personal tablet with the power of an inverted microscope. An intuitive Android- based touch-screen interface is used to control brightfield, three fluorescence channels, and the integratedThe ZOE fluorescent cell imager is a complete imaging system that is suitable for routine cell culture and imaging applications. It eliminates the complexities of cell imaging by combining the ease of use of a personal tablet with the power of an inverted microscope. An intuitive Android- based touch-screen interface is used to control brightfield, three fluorescence channels, and the integrateddigital camera.As a fully integrated system with long-life light-emitting diodes (LEDs), the ZOE cell imager is a robust imaging device ready for intensive daily use. There are no time- consuming setup and hardware adjustments to perform (optical and illumination alignment, changing filter sets or objective lenses) or parts with a limited life span that could result in costly maintenance (replacing mercury arc lamps).The ZOE cell imager uses high-quality hard-coated filter sets that provide high light transmission and long life.Its 20x plan achromatic objective lens is mounted in a proprietary manner, resulting in a wide field of view(0.70 mm2) that is ~180% greater than that of a traditionally mounted 20x objective lens. When zoomed out it is approximately equivalent to that of a 4x objective.When needed, use the pinch-to-zoom gesture to magnify up to 20x by digital zoom while retaining resolution (1 µm).Product features include:■ Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges■ Flexible system — brightfield and three fluorescence channels enable use for routine cell culture applications as well as more sophisticated imaging applications■ Fluorescence at your bench — an integrated light shield permits fluorescence imaging in ambient light■ Robust construction — fully integrated system with long-life LEDs is ready for intensive daily use■ LED light sources — thousands of hours of illumination that are instantly ready after power on■ Large field of view — the motorized stage and large field of view allow you to see more of your sample, faster■ Small footprint — compact size accommodates crowded laboratory benches

Bio-Plex 200 Systems
Más Información

The Bio-Plex> 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample.
Included System Components
• Array reader — combines 2 lasers, high-throughput fluidics, and real-time digital signal processing to distinguish up to 100 different color-coded bead sets, each representing a different assay
• Microplate platform — automates the reading of 96-well plates, yielding up to 9,600 data points in about 35 min
• Calibration and validation kits — system tools for daily calibration of signal output and for IQ/OQ and monthly validation of the primary performance parameters of the reader
• Bio-Plex MCV plate IV — maintenance, calibration, and validation (MCV) plate customized for hands-free startup, shutdown, and performance testing
• PC and monitor — includes Windows 10 operating system, Bio-Plex Manager, Microsoft Office and Adobe Acrobat Reader software, and a 17″ flat-screen monitor»
• Bio-Plex Manager software — controls the instrument, data acquisition, and data analysis for superior curve fitting, statistics reporting, and charting features, without requiring the exporting or importing of files
• Bio-Plex SNP Manager™ data analysis utility — simple-to-use data analysis tool for nucleic acid applications

Bio-Plex 3D Suspension Array System
Más Información
The Bio-Plex 3D suspension array system is the next generation multiplexing platform based on xMAP technology. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for high-throughput testing for nucleic acid and protein applications.
• Measure up to 500 unique analytes in a single sample
• 96- and 384-well plate capability
• Plate read times twice as fast as the Bio-Plex/Luminex 200 system
• Robotics interfacing capabilities
• LIS-compatible software
• Compatible with the magnetic and nonmagnetic assays
• Bio-Plex Manager™ software version 6.0 for data analysis
• Onsite training
Bio-Rad’s Bio-Plex assays can be used with this system. Please check the Bio-Plex 3D Assay Compatibility Chart in the overview before buying assays of interest.
Bio-Plex 3D Instrument

The system comes with all instrument components combined in one shell, a PC, an arm-mounted monitor, keyboard, mouse, sheath fluid, an empty sheath container, a calibration kit and performance verification kit (shipped separately), and an accessory kit with probes, heater blocks, and cables. The system comes with the base version of the xPONENT 4.0 software. As an added value, the system will also come with Bio-Plex Manager software version 6.0 for data analysis.

Optional Upgrades for the Bio-Plex 3D system

Optional Software Modules
All optional modules can be added when purchasing a system. After the purchase of the system, all optional upgrades can be purchased directly through Luminex Corporation.

21CFR Part 11 Module
xPONENT software provides users the opportunity to enable the 21CFR Part 11 module for regulatory compliance. This module maintains data integrity through the use of electronic signatures. The 21CFR Part 11 module contains the Basic Security module.

LIS Module
This module enables xPONENT 4.0 software to transmit and receive data from laboratory information systems (LIS) and laboratory information management systems (LIMS) using an ASTM file format.

Automation Module
The xPONENT 4.0 software facilitates communication/integration between front-end automation devices and Bio-Plex 3D system. The front-end automation provider can build the required driver through an application programming interface (API), which will be supplied with the automation interface module.

Extra Seat Licenses
Extra software seats allow software to be installed on PCs for data analysis away from the instrument. The system comes with one instrument and two desktop PC licenses. Extra seats come in groups of 3. The maximum number of seat licenses per system is 15.

Swivel Base
The system can be purchased with an optional swivel base that allows the unit to rotate by 90° in either direction. This can simplify connection of this system to certain automation platforms. The swivel base can be purchased either at the same time as the instrument or later.

Magnetic Beads and Racks for Immunoprecipitation
Más Información
SureBeads™ Protein G Magnetic Beads and Magnetic Racks are designed for quick and easy immunoprecipitation (IP) and protein complex pull-down. SureBeads Magnetic Beads contain a superparamagnetic core, which enables rapid and highly efficient immunoprecipitation without the need for centrifugation.

 

 

Bio-rad Equipo de Mesa

Benchtop Shaking Incubator Expanded Set
Más Información

Benchtop shaking incubator — handles small batch culture needs easily, for example, growing bacteria or yeast up to 4 L. The incubation temperature can be
set in 0.1° increments up to 65°C. The shaker speed is adjustable between 20 and 300 rpm for periods up to
99 hours or continuous operation. It features outstanding temperature uniformity and stability. Secure a wide variety of vessels with many available accessories.

Temperature-Controlled Water Bath
Más Información

Digital dry bath — digitally controlled dry bath that is ideal for a multitude of laboratory procedures where incubation of samples is needed. It is accurate, built for safe continuous operation, economical, and versatile. Comes with one aluminum alloy 1.5 ml heating block (24 x 1.5 ml capacity). Additional 0.5, 2.0, and 15 ml heating blocks with 24 x 0.5, 24 x 2.0, and 12 x 15 ml capacity, respectively, can be purchased separately; 15 ml dry bath block not shown
Mini incubation oven — can accommodate up to eighty
6.5 cm plates and operates at temperatures up to 60°C. Contains a rear port to allow insertion of our tube roller or mini rocker for temperature-controlled mixing
Water bath — temperature controlled, dependable, affordable water bath that includes stainless steel tank and lid, electrostatically applied finish that resists rust, corrosion, and scratches, over-temperature protection, and thermometer.

Mini Centrifuge, 100–240 V
Más Información

Research-Quality Centrifuges for Classroom Laboratories

Mini Centrifuge

Use this economical and reliable mini centrifuge to quickly spin down samples, pellet cells, or for other similar teaching laboratory applications. This compact unit is provided with a microtube rotor, a PCR strip tube rotor, tube adaptors, and electrical plugs for various regions.

Model 16K Microcentrifuge

Use the Model 16K microcentrifuge for all your teaching laboratory applications. With its brushless motor, this benchtop centrifuge is exceptionally quiet and requires no routine maintenance. Its 18-place rotor to accommodate 1.5 and 2.0 ml tubes.

  • Quick-spin options
  • Compact design
  • Safe for operation in a cold room

For centrifugation of PCR strips or tubes, use the PCR tube adaptor, which is sold separately. This adaptor holds two PCR 8-tube strips or up to 16 individual 0.2 ml tubes. It fits on top of the standard 18-place rotor and is easily attached and removed from the rotor.

Mini Incubation Oven
Más Información

Bio-Rad’s mini incubation oven is simply the most economical and reliable incubator for the growth of bacterial cultures. This high-quality compact oven (0.5 ft3) is thermostatically controlled over a wide temperature range.

Key Features:

  • Thermostatically controlled from ambient to 60°C
  • 80 (6.5 cm) plate capacity
  • Accessory port allows other instruments to be used inside the oven

Baths

Descripción

Heating baths are essential to many experimental workflows. Bio-Rad offers a research-quality water bath and digital dry bath to accommodate your laboratory needs.

Standard Water Bath

This temperature-controlled water bath is built to provide the accuracy, dependability, affordability, and safety required in laboratory applications. The instrument is available with an analog temperature control.

Key features:

  • Easy-to-clean, seamless stainless-steel tank
  • Stainless-steel gable cover
  • Electrostatically applied finish that resists rust, corrosion, and scratches
  • Over-temperature protection
  • Mercury-free thermometer

Digital Dry Bath

This digital dry bath with a stainless steel chamber and aluminum blocks provides rapid and even heating of 0.5 ml, 1.5 ml, or 2.0 ml microcentrifuge tubes or 15 ml centrifuge tubes. The high-wattage heater is microprocessor controlled for precise temperature control. Temperature is set by adjusting the easy-to-read display with the arrow keys. The broad temperature range (ambient +5ºC to 150ºC) is ideal for most common laboratory needs.

Key features:

  • Microprocessor-controlled for precise temperature control
  • Digital display and touch-control setting
  • Includes 1 standard heating block for up to 24 x 1.5 ml microcentrifuge tubes

Additional heating blocks may also be ordered individually:

Temperature-Controlled Water Bath #1660504

Use this temperature-controlled water bath for dependable and affordable laboratory heating from room temperature to 100°C.

  • Easy-to-clean, seamless stainless-steel tank
  • Stainless-steel gable cover
  • Electrostatically applied finish that resists rust, corrosion, and scratches
  • Over-temperature protection
  • Mercury-free thermometer

Digital Dry Bath, 115V #1660562

Use this digital dry bath’s stainless steel chamber and aluminum block for rapid and even heating of samples in standard centrifuge and microcentrifuge tubes. The high-wattage heater is microprocessor controlled for precise temperature control.

  • Includes 1 standard heating block for up to 24 x 1.5 ml microcentrifuge tubes
  • Broad temperature range (ambient +5°C to 100°C) is ideal for most laboratory needs
  • Easy-to-read digital display and touch-control setting
Digital Dry Bath, 115V
Más Información

Utilice la cámara de acero inoxidable y el bloque de aluminio de este baño seco digital para un calentamiento rápido y uniforme de las muestras en tubos de microcentrífuga y centrífuga estándar. El calentador de alto vataje está controlado por un microprocesador para un control preciso de la temperatura.

  • Incluye 1 bloque de calentamiento estándar para hasta 24 tubos de microcentrífuga de 1,5 ml
  • El amplio rango de temperatura (ambiente de +5 °C a 100 °C) es ideal para la mayoría de las necesidades de laboratorio
  • Pantalla digital fácil de leer y configuración de control táctil
Digital Hotplate Stirrer
NO INFO
Model 16K Microcentrifuge
Más Información

220 V, high speed 18-tube benchtop centrifuge, 14,000 rpm (16,000 x g), includes rotor for either 1.5 or 2.0 ml tubes, quick-spin, safe for coldroom, CE-approved.

UltraRocker
Más Información

Bio-Rad’s versatile UltraRocker™ rocking platform meets the demands of many molecular biology applications. The dual platforms are corrosion resistant and are lined with a nonslip rubber mat to keep containers in place. The tilt capacity and variable speed control of this rocker provide a broad range of mixing and agitation options for the following applications:

  • Overnight incubation of liquid bacterial cultures
  • Staining and destaining of agarose and polyacrylamide gels and blots
  • Southern hybridization
BR-2000 Vortexer
Más Información

Use the BR-2000 vortexer for a variety of mixing applications from gentle sample mixing to resuspending cell pellets.

  • Three-position switch allows continuous operation or touch activation
  • Variable speed from 0–3,000 rpm
  • Heavy rubber feet prevent sliding
Mini Rocker, 120 V
Más Información

Use this CE-approved compact rocking platform for a broad range of mixing and agitation in the molecular biology laboratory.

  • Fixed speed (24 rpm)
  • Accommodates up to four 8 x 10 cm or two 10 x 20 cm blotting boxes
  • Fits inside the Bio-Rad mini incubation oven for temperature-controlled experiments
Tube Roller, 120 V
Más Información

The tube roller offers horizontal and vertical mixing angles including end over end mixing, tumbling, rotating, and rocking for samples in a variety of tube sizes. The platform comes with a carousel for 36 x 1.5 ml microcentrifuge tubes and a combination carousel for 10 x 15/10 ml and 16 x 5/7 ml tubes. This compact unit can be used for temperature controlled experiments inside Bio-Rad’s mini incubation oven, which includes an accessory port.

LLamar Ahora
Abrir chat